UDK: 576.851.9:616.981.718

Monoclonal antibodies against antigens of coxiella burnetii: development, characteristics and perspectives of application in laboratory diagnostics of QU fever

E.V. Prokhvatilova, N.P. Khrapova, V.N. Lebedev, S.N. Ionov, V.A. Kharchenko, I.A. Tchourkin,

Волгоградский научно-исследовательский противочумный институт, Вирусологический центр НИИМ МО России, г. Сергиев-Посад, Московская обл. ФГУЗ Центр эпидемиологии и гигиены по Волгоградской обл.

Abstract

The collection of hybridomas (7 types) producing monoclonal antibodies (MA) by fusion of cells of mice myeloma of line P3-X63-Ag.653 and splenocytes of BAL/c mice, immunized with antigens of I-II phase of C.burnetii has been developed. MA are directed at different epitomes, located on the antigen complexes of Coxiella burnetii. The char acteristics of MA (specifity, activity) have been studied using ELISA and FIA; perspectives of their application for the laboratory diagnostics of QU fever are discussed.

Keywords

monoclonal antibodies, coxiella burnetii, laboratory diagnostics

Contacts

Волгоградский научно-исследовательский противочумный институт, vari@sprint-v.com.ru