UDK: 615.015.12: 615.252.349.7: 57.088.1

OPTIMIZATION OF METHOD IN VITRO FOR RE-GLYCATION ACTIVITY OF COMPOUNDS INVESTIGATION

A. A. Spasov1,2, A. I. Rashchenko1, 3, A. A. Brigadirova1,2

1Волгоградский государственный медицинский университет, кафедра фармакологии, 2Волгоградский медицинский научный центр, лаб. экспериментальной фармакологии, 3НИИ фармакологии, лаб. антиоксидантных средств

Abstract

We reproduced an experimental model of studying in vitro re-glycation activity of compounds. The reliability of this method was confirmed be means of the tests employing alagebrium, a well-known advanced glycation end products (AGEs) breaker,in concentrations from 7 to 1,25 mM. The half-maximal inhibitory concentration (IC50) of this substance was calculated.

Keywords

glycation, advanced glycation end products (AGEs), diabetes mellitus, alagebrium, aminoguanidine, model in vitro.

Contacts

Бригадирова Анастасия Андреевна — ассистент каф. фармакологии ВолгГМУ, м. н. с. лаб. экспериментальной фармакологии ГБУ ВМНЦ, e-mail: a.brigadirova@gmail.com