UDK: 611.813.3.018:616.8-008.615:599.323.4

MORPHOFUNCTIONAL CHANGES OF THE DORSAL AND VENTRAL HIPPOCAMPUS OF RATS IN MODELING THE COMBINED STRESS WITH CASPASE-3 AND GFAP EXPRESSION

A.V. Smirnov1,4, N.V. Grigorieva1, M.R. Ekova1, M.V. Schmidt1,4, D.S. Mednikov1, I.N Tyurenkov2, D.V. Kurkin2, E.V. Volotova2, V.S. Zamaraev3

ФГБОУ ВО «Волгоградский государственный медицинский университет» Министерства здравоохранения Российской Федерации, 1кафедра патологической анатомии, 2кафедра фармакологии и биофармации ФУВ, 3кафедра микробиологии, вирусологии, иммунологии с курсом клинич

Abstract

The dorsal hippocampus (DH) is responsible for certain forms of learning and memory, and the ventral hippocampus (VH) plays an important role in the realization of stress reactions and anxious forms of behavior. The study was performed on 20 white rats at the age of 12 months. Modeling of combined stress (pulsating bright light, loud sound, vibration) was conducted daily for 30 minutes for 7 days in a special chamber consisting of 6 isolated compartments. In stressed animals (n = 10), in the DH and VH, a decrease the area of the neuronal perikarya (ANP), an increase in the relative number of shrunken neurons with hyperchromatosis of the cytoplasm, an increase in the relative area of GFAP immunoreactive material was observed. In addition, the decrease in relative ANP, width of the pyramidal layer, and the increase in the relative amount of caspase-3- immunopositive neuronal perikarya were observed in DH. With prolonged combined stress in rats at the age of 12 months, the most pronounced changes in the pyramidal layer in the DPH are found in CA3, and in the VH – in CA1.

Keywords

hippocampus, caspase-3, GFAP, stress, rat.

Contacts

Экова Мария Рафаэлевна – ассистент кафедры патологической анатомии, Волгоградский государственный медицинский университет, e-mail: maria.ekova@mail.ru