UDK: 576.581.132-616.982.27-039

Phenotypic characterization of Burkholderia mallei mutants defected in the synthesis of extracellular proteases

Ageeva N.P., Merinova L.K., Victorov D.V., Plehanova N.G.

Волгоградский научно-исследовательский противочумный институт

Abstract

Strains of B. mallei, defective in production of extracellular enzymes were isolated using transposone mutagenesis method. Mutants were been characterized by activity of extracellular enzimes, virulence and some other properties. The method of electrophoresis in SDS – PAAG and immunoblotting were imployed for analysing of protein profiles. It was revealed that composition and quantity of protein accounted for changes of proteolytic activity.

Keywords

Contacts

Волгоградский научно-исследовательский противочумный институт, vari@sprint-v.com.ru