UDK: 612.117.1/612.117.7
E.A. Sladkova1, N.I. Zhernakova2, M.Yu. Skorkina1, A.A. Grishenko2
ФГАОУ ВО «Белгородский государственный национальный исследовательский университет», Медицинский институт, 1кафедра биохимии; 2кафедра семейной медицины
Erythrocytes’ membranes are unique subjects allowing tracing the changes of the ultrastructure and biophysical properties of membrane ageing. The goal of this study is the investigation of the features of the ultrastructural organization and biophysical properties of the membrane’s erythrocyte in the elderly people. In the work was used the method of atomic force microscopy for the estimation of the relief and elastic properties, electrical and adhesive properties of a cell’s surface. In the results of the study has been established the availability of a few morphological formations in the form of globular structures and deepening on the surface’s erythrocyte of the elderly people. For the erythrocytes were the negative charge of membrane (-49,4 ± 3,8) mV, Young’s module was (3,94 ± 0,2) mPа. It was measured the adhesive forces in the system «erythrocyte – erythrocyte» (27,9 ± 0,6) nN and in the system «erythrocyte – leukocyte» (59,4 ± 0,5) nN. The obtained data open up new opportunities for the development of the fundamentally new approaches in the study of functional properties of cells’ plasmalemma on the various stage of ontogenesis.
membrane’s erythrocyte, atomic force microscopy, ultrastructure of a surface, adhesion, elastic properties, surface potential.
Сладкова Евгения Анатольевна – к. м. н., доцент кафедры биохимии, медицинский институт, Белгородский государственный национальный исследовательский университет; e-mail: sladkova@bsu.edu.ru