UDK: 616-08;615.9:623.459;615

EFFECT OF HUMAN HIGHLY PURIFIED BUTIRYLHOLINESTERASE OF CONJUGATED WITH COLLOID GOLD ON B-ESTERASE ACTIVITY AFTER EXPOSURE BY DIISOPROPYL FLUOROPHOSPHATE

Yu.I. Velikorodnaya1, V.A. Antonov1, A.Ja. Pocheptsov1, I.A. Dvoryashina1,2

1ФГУП «Научно-исследовательский институт гигиены, токсикологии и профпатологии» Федерального медико-биологического агентства; 2ФГБОУ ВО «Волгоградский государственный медицинский университет» Министерства здравоохранения Российской Федерации, кафедра гист

Abstract

The use of butyrylcholinesterase immobilized on nanoparticles as a means of preventing acute poisoning with highly toxic organophosphates at a dose LD50 in experiments in vivo was studied. Entering of the drug 24 hours before exposure to diisopropyl fluorophosphate prevented the development of symptoms of acute intoxication and protected mice from death. In addition, the prophylactic administration of butyrylcholinesterase conjugated to colloidal gold significantly slowed the decrease in the activity of butyrylcholinesterase and acetylcholinesterase in the blood plasma and had a protective effect on cholinesterase in the brain.

Keywords

cholinesterase, highly purified cholinesterase, colloidal gold, organophosphorus compound, prevention.

Contacts

Великородная Юлия Ивановна – зав. лабораторией патоморфологии, ФГУП Научно- исследовательский институт гигиены, токсикологии и профпатологии ФМБА России, e-mail: velikorodnaya@rihtop.ru