UDK: 577.29:615

DEVELOPMENT OF THE TECHNIQUE FOR DETERMINATION OF IMPURITIES IN SUBSTANCE OF THE NEW COMPOUND WITH AN ANTIARRHYTHMIC ACTIVITY – ALM-802

L.N. Grushevskaja, V.V. Adzhienko, M.S. Sergeeva, L.M. Gaevaja, M.E. Dudenkova, E.D. Denisenko, N.I. Avdjunina, S.V. Minaev, G.V. Mokrov

ФГБНУ «Научно-исследовательский институт фармакологии имени В.В. Закусова»

Abstract

A technique for determining related impurities in the substance of a new compound with antiarrhythmic activity – ALM-802 has been developed. Separation of ALM-802 and impurities was carried out by reverse phase high performance liquid chromatography (HPLC) on a BDS Hypersil 150 x 4.6 mm, 5 μm column (Thermo Scientific) in gradient elution mode. Mixtures of a 0,02 M potassium dihydrogen phosphate solution, methanol and acetonitrile were used as the mobile phase. Spectrophotometric detector, analytical wavelength 205 nm. A number of unidentified impurities were found in the experimental sample of ALM-802, the synthesis product, 2,3,4-trimethoxybenzaldehyde, was not found.

Keywords

ALM-802, related impurities, high-performance liquid chromatography.

Contacts

Гаевая Людмила Михайловна – д. фарм. н., ведущий научный сотрудник химико-технологической лаборатории опытно-технологического отдела ФГБНУ «НИИ фармакологии имени В.В. Закусова», e-mail: otopharm@mail.ru