UDK: 615.22-099:616-092.4
V.K. Shormanov, L.L. Kvachakhiia, D.P. Shcherbakov
ФГБОУ ВО «Курский государственный медицинский университет» Министерства здравоохранения Российской Федерации
A study was made of the preservation features of diltiazem in putrid decaying biological material (liver tissue) under three temperature regimes: 18–22; 8–10; 1,5–2 °C. The test substance was isolated from the model mixtures with biological material by infusion with acetone twice for 30 minutes. Purification of the recovered compound was carried out by chromatography on a column of «Silasorb S-18» 30 μm (eluent – «acetonitrile-water» (9,1:0,9 by volume). Optimal conditions for identification and quantitative determination of diltiazem extracted from putrefied tissue liver by thin layer chromatography (TLC), gas chromatography – mass spectrometry (GC-MS) and spectrophotometry in the UV- and visible regions of the spectrum. It is established that with a decrease in temperature from 22 to 1,5–2 °C, the duration of the preservation of the substance increases from 100 to 140 days.
diltiazem, isolation, purification, determination, preservation, biological material, chemical-toxicological analysis.
Шорманов Владимир Камбулатович – д. фарм. н., профессор кафедры фармацевтической, токсикологической и аналитической химии, Курский государственный медицинский университет, e-mail: R-WLADIMIR@yandex.ru