UDK: 582.998.1:547.913:543.544.943.3

DEVELOPMENT AND VALIDATION OF METHODS FOR DETERMINATION OF CAPILLIN IN ESSENTIAL OIL OF ARTEMISIA SCOPARIA

E.E. irapetyan1, T.D. Mezenova1, .M. Shevchuk2, L. . Logvinenko2, D. . Konovalov1

1Пятигорский медико-фармацевтический институт – филиал ФГБОУ ВО «Волгоградский государственный медицинский университет» Министерства здравоохранения Российской Федерации, кафедра фармакогнозии, ботаники и технологии фитопрепаратов; 2ФГБУН «Ордена трудовог

Abstract

The method of identification and quantitative determination of capillin content in essential oil from the herb of Artemisia scoparia has been developed and validated. The method of chromatography in a thin layer of a sorbent with densitometric registration of analytical signal is applied. For the experiment, the essential oil of Artemisia scoparia obtained by hydrodistillation was used. Digital analysis of chromatograms was performed using the program «Videodensitometr Sorbfil». For chromatography used plates of the brand «Sorbfil» PTSX-P-A-UV, mobile phase-hexane-ethyl acetate (9:1). In daylight, four different intensity yellow spots appear on the chromatogram. Identified: capillol (Rf 0,29), capilline (Rf 0,61), capillen (Rf 0,80). Their content in the essential oil, as determined by the method of internal normalization in the experiment, is: capillol – 20,6 %, capilline – 47,8 %, capillen – 26,1 %. Validation of methodology was carried out according to its specificity (Rf capillina corresponds to Rf standard sample); sensitivity - limit of detection (PО = 13.7 mkg), the quantification limit (PKO = 41.6 mkg); linearity (the regression equation has the form: S = 1,1.103 m); the correctness of the method «introduced-found» (Хср = 103,7 %; Х = 9,1 %; RSD % = 9,9 %; Е = 8,8 %); precisionist on 6 samples analyte (Хср = 9,65 %; Х = 1,00 %; RSD % = 9,9 %; Е = 10,4 %).The method is planned to be used to control the quality of the medicinal plant and to study the variability of capillin content in the samples of essential oil from the herb of Artemisia scoparia of different geographical origin.

Keywords

capillin, essential oil, herbа of Artemisia scoparia, thin-layer chromatography, qualitative, quantitative determination, validation.

Contacts

Коновалов Дмитрий Алексеевич – д. фарм. н., профессор кафедры фармакогнозии, ботаники и технологии фитопрепаратов, Пятигорский медико-фармацевтический институт – филиал ФГБОУ ВО ВолгГМУ Минздрава России, e-mail: konovalov_da@pochta.ru