UDK: 615.072
R.A. Litvinov, V.A. Kosolapov, E.A. Muravyova, I.V. Skachko, D.D. Shamshina
ФГБОУ ВО «Волгоградский государственный медицинский университет» Министерства здравоохранения Российской Федерации, лаборатория метаботропных лекарственных средств НЦИЛС ВолгГМУ
The article considers a particular model of the glycation reaction, called glycoxidation and characterized by a significant involvement of oxidative reactions and the participation of transition metals. The enhancement of oxidative processes, which makes it possible to identify the reaction as glyoxidation, was realized by introducing copper (II) cations (CuSO4 * 5H2O) into the reaction medium in a form accessible for interaction with the reaction participants. The main reagents in the glycoxidation reaction, in addition to CuSO4*5H2O are bovine serum albumin (1 g/l), glucose (0,5 M), HEPES buffer solution (24 g/l) dissolved in deionized water. The conditions have been selected under which the glycoxidation reaction linearly depends on the concentration of copper (II), which makes the cation activity a sensitive target when monitoring the reaction. The dependence of the reaction on the concentration of CuSO4 * 5H2O under the presented experimental conditions has been confirmed experimentally and mathematically. An analytical range of concentrations of CuSO4*5H2O has been established, in which a linear dependence of AGE formation on concentration is observed. For the concentration of CuSO4*5H2O, which produces the greatest potentiating effect on the AGE formation (10 mg/l), the procedure was validated by the indices of internal laboratory reproducibility and convergence. The technique is of interest for investigation of the antiglycoxidative activity of novel compounds.
Glycoxidation, CuSO4*5H2O, HEPES, bovine serum albumin, glucose, glycation end products (AGE).
Литвинов Роман Александрович – к. м. н., доцент кафедры фармакологии и биоинформатики, с. н. с. лаборатории метаботропных лекарственных средств НЦИЛС, Волгоградский государственный медицинский университет, e-mail: litvinov.volggmu@mail.ru