UDK: 576.315:611.018.73:615.834

INFLUENCE OF SPELEOCLIMATOTHERAPY ON THE PSYCHO-EMOTIONAL STATE, GENETIC STABILITY AND ACTIVITY OF α-AMYLASE IN THE ORAL CAVITY OF THE STUDENTS

M.S. Nechaeva, O. I. Tyunina, E.V. Dorokhov, A.Yu. Aralova, Yu.O. Potapova

ФГБОУ ВО «Воронежский государственный медицинский университет им. Н.Н. Бурденко» Министерства здравоохранения Российской Федерации, кафедра нормальной физиологии

Abstract

The goal of investigation was to evaluate the effect of speleoclimate on the psycho-emotional state, the frequency of occurrence of buccal epithelial cells with core aberrations and the level of α-amylase activity in the oral cavity of students. Second-year students of VSMU named N. N. Burdenko were selected as subjects. The collection of psychological parameters, buccal epithelium, to assess genetic stability, and oral fluid, for evaluation of the activity of α-amylase was carried out in the first, fourth, eighth, eleventh day visit speleochamber and four days after a course of speleoclimate. The psychoemotional state of the subjects was assessed using the SAN questionnaire and the scale of C.D. Spielberger and Y.L. Khanin. The state of genetic homeostasis of students was evaluated using a micronucleus test in the buccal epithelium. The level of activity of α-amylase was carried out by the method of solid-phase enzyme immunoassay. Comparison of the studied indicators was carried out using the nonparametric Van-der-Warden criterion. The relationship between the dynamics of α-amylase activity and cytological parameters was revealed using the Spearman correlation coefficient (rs). The results obtained indicate a positive effect of speleoclimate on the psychological and genetic stability of a person. Subjects with low α-amylase activity showed a higher level of cells with abnormal nuclei. A negative correlation was found between the dynamics of the frequency of abnormal nuclei in buccal epithelial cells and the activity of α-amylase in the group with initially low activity (rs= – 0,8; P < 0,05). Speleoclimatotherapy can be recommended as a method of non-medicamental correction of the effects of stress influence on health of students.

Keywords

micronucleus test, core abnormalities, α-amylase, psycho-emotional state, students' health.

Contacts

Дорохов Евгений Владимирович – к. м. н., доцент, зав. кафедрой нормальной физиологии, Воронежский государственный медицинский университет имени Н.Н. Бурденко, e-mail: dorofov@mail.ru