UDK: 577.21:616.006

ANALYSIS OF METHYLATION STATUS ANALYSIS OF RASSF1A, BRCA1, HIN1 IN BREAST AND OVARIAN CANCER CELL LINES

A. A. Potapova, O. V. Ostrovskiy, G. P. Dudchenko

Кафедра теоретической биохимии с курсом клинической биохимии ВолГМУ, Филадельфийский онкологический центр*

Abstract

Several tumor suppressor genes have been found to be hypermethylated in many tumor types, including breast and ovarian cancer but to be unmethylated in normal cells. Here we have examined by bisulfite sequencing the methylation status of the following tumor-suppressor genes:RASSF1A, BRCA1 and HIN1. For this study we used genomic DNA from five different breast cancer cell lines (MDA231, MDA435, MCF7, T47D, HS-578T) and five different ovarian cancer cell lines (A2780, SKOV3, OVCAR10, OVCAR5, OVCAR3) as well as from normal tissue. We found promoter region of RASSF1A and HIN1 to be hypermethylated in several cancer cell lines in both types of cancer. At the same time the promoter region of BRCA1 was unmethylated in all analyzed cancer cell lines.

Keywords

methylated gene, unmethylated gene, RASSF1A, BRCA1, HIN1, breast cancer, ovarian cancer

Contacts

Островский Олег Владимирович — д. м. н., про- фессор, зав. каф. теоретической биохимии с курсом клинической биохимии, e-mail: ol.ostr@gmail.com