UDK: 576.8.097.3:576.851.132

DIFFERENTIAL EXPRESSION OF CYTOKINE GENES IN MICE INFECTED BY BURKHOLDERIA PSEUDOMALLEI AND B. THAILANDENSIS

S. V. Perepelitsyna, V. V. Alekseev, I. B. Zakharova, N. G. Plekhanova, D. V. Viktorov

Волгоградский научно-исследовательский противочумный институт, Волгоградский государственный медицинский университет

Abstract

Using RT-PCR technique, the differences in cytokine genes expression in mice infected by Burkholderia pseudomallei и B. thailandensis strains were investigated. The maximal mRNA level of IFN- and IL-12, belongs to Th1-type cytokine profile of immune responce, was revealed in mice infected by avirulent B. thailandensis. The acute melioidosis caused by B. pseudomallei 100, contrastly, was characterized by termination of IFN- and IL-12 production and increase of IL-10 mRNA quantity in latest stages of observation. The transcription profiles of cytokine genes in mice infected by attenuated mutant B. pseudomallei 56770 99/10 was more related to case of B. thailandensis, however apparent similarity was matched in early stages of infection only. The obtained results demonstrate clear differences in immune response during infection caused by B. pseudomallei and B. thailandensis, аnd a given approach may be useful to investigate the traits of Burkholderia pathogenesis on molecular level.

Keywords

Burkholderia pseudomallei, Burkholderia thailandensis, cytokine, mRNA, differential expression

Contacts

Перепелицына Светлана Валерьевна — научный сотрудник лаборатории аэрогенного заражения ФГУЗ ВолгоградНИПЧИ Роспотребнадзора, e-mail: vari2@sprint-v.com.ru