UDK: 616.074

OPTIMIZATION OF THE PROTOCOL OF DETERMINING THE LEVEL OF CELL-FREE DNA IN PLASMA

A. A. Gneusheva, V. E. Verovskiy, O. V. Ostrovskiy

Волгоградский государственный медицинский университет, кафедра теоретической биохимии с курсом клинической биохимии

Abstract

We adapted the protocol of measuring cell-free DNA (cfDNA) level in plasma for application in clinical practice. This protocol is based on sorption extraction of DNA followed by measuring its concentration with a Qubit fluorometer. We explored the analytical parameters of this procedure. The regional reference limit of the cfDNA level in plasma of healthy volunteers was 14,7 ng/ml to 104,7 ng/ml.

Keywords

cell-free DNA.

Contacts

Гнеушева Александра Андреева — аспирант кафедры теоретической биохимии с курсом клинической биохимии Волгоградского государственного медицинского университета, e-mail: alexa-1808@yandex.ru