UDK: 620.3:57.085.23; 615.916

EFFECT OF GOLD NANOPARTICLES ON THE VIABILITY AND METABOLIC PARAMETERS OF CELL LINES OVER TIME

Yu. I. Velikorodnaya1, A. Ya. Pocheptsov1, I. A. Dvoryashina1,2, V. L. Zagrebin2, O. I. Sokolov3

1ФГУП «Научно-исследовательский институт гигиены, токсикологии и профпатологии» ФМБА России, Волгоград, 2Волгоградский государственный медицинский университет, кафедра цитологии, гистологии и эмбриологии, 3ФГБУН «Институт биохимии и физиологии растений и

Abstract

We studied the response of cellular systems to the introduction of 5 nm colloidal gold 4, 24 and 48 hours, and 24 hours after replacing the growth medium containing gold nanoparticles. We evaluated the activity of acid phosphatase and lactate dehydrogenase in the supernatant. We also performed a cytotoxic test. The results showed that the damaging effect of gold nanoparticles on the cells was reversible, and metabolic changes were condiioned by adaptive mechanisms.

Keywords

gold nanoparticles, cytotoxicity, lactate dehydrogenase, acid phosphatase, recover.

Contacts

Великородная Юлия Ивановна — научный сотрудник лаборатории патоморфологии, ФГУП Научно-исследовательский институт гигиены, токсикологии и профпатологии ФМБА России, e-mail: alta-u@mail.ru