UDK: 615.072

OPTIMISATION OF COPPER-INDUCED ASCORBATE AUTOXIDATION METHOD USING REAL-TIME DETECTION. INTEGRATIVE DECISION EXAMPLE

A.A. Ivanov, D.D. Shamshina, R.A. Litvinov, V.V. Batrakov

ФГБОУ ВО «Волгоградский государственный медицинский университет» МЗ РФ

Abstract

This paper describes the new approach of process of optimization and comprehended analysis of results for copperinduced ascorbate autoxidation method. This is a metod of chelating activity detection. Real-time technique in data registration was used and integrative analysis in comparison of the results was applied. It gived a possible to evaluate the chelation properties of pioglitazone without calculation of IC50. This variant is most acual for spread screening of chelating activity.

Keywords

ascorbate, multifunctional autoxidation, chelation, copper sulfate (II), real-time analysis.

Contacts

Литвинов Роман Александрович – к. м. н., ассистент кафедры фармакологии, Волгоградский государственный медицинский университет, e-mail: litvinov.volggmu@mail.ru