UDK: 577.1.01

DEVELOPMENT AND VALIDATION OF THE TECHNIQUE DEFINITION OF THE INDICATOR «RESIDUAL ORGANIC SOLVENTS» IN SUBSTANCE GSB-106

M.E. Dudenkova, L.N. Grushevskaja, M.S. Sergeeva, L.M. Gaevaja, E.D. Denisenko, S.V. Minaev, A.V. Tarasjuk

ФГБНУ «Национальный научно-исследовательский институт фармакологии имени В.В. Закусова»

Abstract

The technique of identification and quantitative determination definition of residual organic solvents in the original substance synthesized in FSBSI «National Research Institute of Pharmacology named after V.V. Zakusov» on the basis of structure of the fourth loop of BDNF (GSB-106) is developed using method of gas-liquid chromatography (GLC) with static vapor-phase extraction. The developed conditions of analysis allowed to effectively divide chromatographic peaks of all organic solvents used in the course of synthesis and purification of substance at the last stages: methanol, pyridine, ethanol, acetic acid. The validation of the technique proved its specificity, linearity, correctness.

Keywords

GSB-106, residual organic solvents, gas-liquid chromatography, vapor-phase extraction, validation.

Contacts

Дуденкова Мария Евгеньевна – к. ф. н., старший научный сотрудник опытно-технологического отдела ФГБНУ «Национальный НИИ фармакологии имени В.В. Закусова», e-mail: otopharm@mail.ru