Journal of VolgSMU
Quarterly scientific-practical journal

UDK: 615:547.466.3:616.36+576.311.347

EFFECT OF GABA AND GLUTAMIC ACID DERIVATIVES ON THE FUNCTIONAL ACTIVITY OF LIVER CELL MITOCHONDRIA IN VITRO UNDER CONDITIONS OF OXIDATIVE STRESS

G.H. Khusainova, T.A. Popova, M.V. Kustova, O.V. Ostrovsky

Волгоградский государственный медицинский университет, Волгоград, Россия

Abstract

The aim of the study was to study the effect of GABA derivatives on the functional activity of intact and oxidative stress-damaged mitochondria. Isolated mitochondria were obtained by differential centrifugation. Oxidative damage was simulated by incubation with tert-Butylhydroperoxide (200 nmol/ml, 10 min). The studied substances (baclofen, mefebut, neuroglutam, RGPU-238 compound, salifen, tolibut, phenibut, phenotropil) were added to intact and damaged mitochondria (1×10-5 M per 100 μl, 10 min of incubation), the functional state of mitochondria was studied using a polarographic method. It was revealed that salifen and phenotropil had a pronounced protective effect on the respiratory complexes of the electron transport chain.

Keywords

GABA derivatives, glutamic acid derivatives, reactive oxygen species, mitochondrial respiration

Contacts

Гульнара Хамзаевна Хусаинова, Gulnarahusainova130@gmail.com